Written in English
|Statement||by John Ormond Cisar.|
|The Physical Object|
|Pagination||, 136 leaves, bound :|
|Number of Pages||136|
Abstract. Graduation date: After parenteral immunization with Aeromonas salmonicida\ud cells, the agglutination activity in juvenile coho salmon (Oncorhynchus\ud kisutch) serum was associated with the macroglobulin serum fraction.\ud Antibody activity was diminished by 30 minutes exposure to 50 C\ud but not 45 C. Reduction with 2-mercaptoethanol and alkylation\ud markedly lowered but did. Abstract. Coho salmon (Oncorhynchus kisutch) anti-Aeromonas salmonicida agglutinins were excluded from Sephadex G and their activity was lost after partial reduction and alkylation followed by exposure to 2 M electrophoretic analysis of coho antiserum revealed anodic and cathodic populations of immunoglobulin (Ig), and the anti-A. salmonicida antibodies present 12 Cited by: Specific antibody production in response to an intraperitoneal injection of A. salmonicida endotoxin first occurred in juvenile coho salmon (Oncorhynchus kisutch) held at , , and C at. 1. Infect Immun. Feb;9(2) Characterization of anti-Aeromonas salmonicida antibodies from coho salmon. Cisar JO, Fryer JL. Coho salmon (Oncorhynchus kisutch) anti-Aeromonas salmonicida agglutinins were excluded from Sephadex G and their activity was lost after partial reduction and alkylation followed by exposure to 2 M urea.
This ELISA method could detect the antibody in sera of masu (Oncorhynchus masou), chum (O. keta) and coho salmon (O. kisutch) immunized by A. salmonicida and could be applied to detect the. Detection of antibody against Aeromonas salmonicida in the serum of salmonid fish by the enzyme linked chum (0. ktlu) and coho salmon (0. klslIll'h) immunized by A. safmonicida and could be applied to detect the antibody against times and SO 1'/ or the rabbit anti-masu salmon Ig (diluted: in PBSTween) was added. Stephen L. Kaattari, Jon D. Piganelli, in Fish Physiology, 2. Aeromonas salmonicida. Aeromonas salmonicida, causative agent of furunculosis in salmonids, is a Gram-negative, nonmotile rod (Fig. 3).It is a pathogen of both salmon and trout and is endemic to North America, Europe, and Japan. Antibody response in rainbow trout (Salmo gairdneri). II. Studies on the kinetics of development of antibody-producing cells and on complement and natural hemolysin. J. Immunol., Cisar, J.O., Properties of anti-Aeromonas salmonicida antibodies from juvenile coho salmon (Onchorynchus kisutch). Dissertation, Oreg.
Aeromonas salmonicida subsp. salmonicida (hereafter A. salmonicida) is the aetiological agent of furunculosis in marine and freshwater A. salmonicida invade the fish host through skin, gut or gills, it spreads and colonizes the head kidney, liver, spleen and brain.A. salmonicida infects leucocytes and exhibits an extracellular phase in the blood of the host; however, . Immune response of Cryptobia-resistant and Cryptobia-susceptible Salvelinus fontinalis to an Aeromonas salmonicida vaccine production In coho salmon the antibody response of juvenile coho. The antibody response of a population of juvenile coho salmon (Oncorhynchus kisutch) held at 12 C approached % following a single parenteral vaccination with Aeromonas salmonicida cells. This antibody recognizes typical (ATCC ) and atypical strains of Aeromonas salmonicida and can be used in ELISA (1: 4, dilution), Western Blot (1: 1, dilution) and indirect immunofluorescence (1: 1, dilution) assays. A. salmonicida is a Gram-negative, non-motile, rod-shaped and faculta.